验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, K-562 cells, mouse brain tissue, rat brain tissue |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy DDX3 Ready-To-Use IHC Kit. DDX3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
81903-1-RR targets DDX3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF/ICC, FC (Intra), ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | DDX3 fusion protein Ag1614 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked |
| Synonyms | DDX3X, DDX14, DBX, CAP-Rf, ATP-dependent RNA helicase DDX3X |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC011819 |
| Gene Symbol | DDX3 |
| Gene ID (NCBI) | 1654 |
| RRID | AB_2935591 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O00571 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DDX3X, also named as DBX, DDX3 and HLP2, belongs to the DEAD box helicase family and DDX3/DED1 subfamily. It is ATP-dependent RNA helicase. DDX3X acts as a cofactor for XPO1-mediated nuclear export of incompletely spliced HIV-1 Rev RNAs. It is also involved in HIV-1 replication. DDX3X interacts specifically with hepatitis C virus core protein resulting in a change in intracellular location. 73 kDa is the target band. 60-65 kDa is some other isoform. This antibody can bind both DDX3X and DDX3Y for the close sequences.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX3 antibody 81903-1-RR | Download protocol |
| IF protocol for DDX3 antibody 81903-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |