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G3BP2 Recombinant antibody, PBS Only (Capture)

G3BP2 Recombinant Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

230275D6

Cat No : 82080-4-PBS

Print datasheet

Synonyms

G3BP 2, G3BP2, KIAA0660

验证数据展示

  • WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

    WB analysis using 82080-4-RR (same clone as 82080-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82080-4-RR (G3BP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

    IF Staining of HeLa using 82080-4-RR (same clone as 82080-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP2 antibody (82080-4-RR, Clone: 230275D6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82080-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00090-1

    Cytometric bead array standard curve of MP00090-1

    Cytometric bead array standard curve ofMP00090-1, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-3-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00090-2

    Cytometric bead array standard curve of MP00090-2

    Cytometric bead array standard curve ofMP00090-2, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-5-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve of MP00090-3

    Cytometric bead array standard curve ofMP00090-3, G3BP2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82080-4-PBS. Detection antibody: 82080-2-PBS. Standard: Ag9355. Range: 0.625-20 ng/mL.

  • Affinity experiments of 82080-4-RR

    Affinity experiments of 82080-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82080-4-RR against Human G3BP2 were performed. The affinity constant is 59.6 nM.

Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.

产品信息

82080-4-PBS targets G3BP2 as part of a matched antibody pair:

MP00090-1: 82080-4-PBS capture and 82080-3-PBS detection (validated in Cytometric bead array)

MP00090-2: 82080-4-PBS capture and 82080-5-PBS detection (validated in Cytometric bead array)

MP00090-3: 82080-4-PBS capture and 82080-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen G3BP2 fusion protein Ag9355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 2
Synonyms G3BP 2, G3BP2, KIAA0660
Calculated Molecular Weight482aa,54 kDa; 449aa,51 kDa
GenBank Accession NumberBC011731
Gene Symbol G3BP2
Gene ID (NCBI) 9908
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UN86
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.