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验证数据展示

  • WB analysis using 82441-1-RR

    WB analysis using 82441-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82441-1-RR (Cystatin C antibody) at dilution of 1:3300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 82441-1-RR

    IHC staining of human kidney using 82441-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 82441-1-RR

    IHC staining of human kidney using 82441-1-RR

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 82441-1-RR (Cystatin C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HepG2 using 82441-1-RR

    WB analysis of HepG2 using 82441-1-RR

    WB result of Cystatin C antibody (82441-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cystatin C transfected HepG2 cells.

  • Affinity and Kinetic Characterization of 82441-1-RR

    Affinity and Kinetic Characterization of 82441-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82441-1-RR against Human Cystatin C were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

Cystatin C Recombinant antibody

Cystatin C Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82441-1-RR

产品说明书

CloneNo. 1I20

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

CST3, 1I20, Gamma-trace, Cystatin-C, Cystatin-3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, human milk, human urine
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:15000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82441-1-RR targets Cystatin C in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 Cystatin C fusion protein Ag2890 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cystatin C
别名 CST3, 1I20, Gamma-trace, Cystatin-C, Cystatin-3
计算分子量 146 aa, 16 kDa
观测分子量 13 kDa
GenBank蛋白编号BC013083
基因名称 Cystatin C
Gene ID (NCBI) 1471
RRIDAB_3086479
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01034
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

实验方案

Product Specific Protocols
WB protocol for Cystatin C antibody 82441-1-RRDownload protocol
IHC protocol for Cystatin C antibody 82441-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols