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验证数据展示

  • IF Staining of mouse kidney using CL488-82441

    IF Staining of mouse kidney using CL488-82441

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 488 Cystatin C antibody (CL488-82441, Clone: 1I20 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse kidney using CL488-82441

    IF Staining of mouse kidney using CL488-82441

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using CoraLite® Plus 488 Cystatin C antibody (CL488-82441, Clone: 1I20 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Cystatin C Recombinant antibody

Cystatin C Uni-rAbTM Recombinant Antibody for IF-P
Cat No. CL488-82441
CloneNo. 1I20

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF-P

Synonyms

ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse kidney tissue
For other applications, we recommend the unconjugated version of this antibody, 82441-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82441 targets Cystatin C in IF-P applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, mouse
Immunogen Cystatin C fusion protein Ag2890 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cystatin C
Synonyms ARMD11, CST3, Cystatin 3, cystatin C, Gamma trace, Post gamma globulin
Calculated Molecular Weight 146 aa, 16 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC013083
Gene Symbol Cystatin C
Gene ID (NCBI) 1471
RRIDAB_3673064
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01034
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin C is a 13-kDa inhibitor of cysteine proteinases which is secreted by all cell types and is completely cleared from the organism through glomerular filtration, shown to be an early and sensitive biomarker of renal dysfunction. It is also used as an emerging biomarker in cardiovascular disease. Cystatin C is involved in a variety of inflammatory reactions. The concentration of serum cystatin C has also been shown to be unaltered in certain inflammatory conditions or other disorders of metabolism. The plasma level of serum cystatin C can be expressed as its level of generation from cells and diet and its subsequent elimination through the gut, liver, and kidneys.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cystatin C antibody CL488-82441Download protocol
Standard Protocols
Click here to view our Standard Protocols