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验证数据展示

  • WB analysis using 82463-1-RR

    WB analysis using 82463-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82463-1-RR

    IP experiment of HeLa using 82463-1-RR

    IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug.

  • IHC staining of rat small intestine using 82463-1-RR

    IHC staining of rat small intestine using 82463-1-RR

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VCP antibody included
  • IF Staining of rat brain using 82463-1-RR

    IF Staining of rat brain using 82463-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82463-1-RR

    IF Staining of HeLa using 82463-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82463-1-RR

    Affinity and Kinetic Characterization of 82463-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.

VCP Recombinant antibody

VCP Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 82463-1-RR

产品说明书

CloneNo. 3G23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, RAW 264.7 cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy VCP Ready-To-Use IHC Kit. VCP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82463-1-RR targets VCP in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VCP fusion protein Ag1002 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 valosin-containing protein
别名 HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit
计算分子量 89 kDa
观测分子量89 kDa
GenBank蛋白编号BC007562
基因名称 VCP
Gene ID (NCBI) 7415
RRIDAB_3086480
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP55072
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

实验方案

Product Specific Protocols
WB protocol for VCP antibody 82463-1-RRDownload protocol
IHC protocol for VCP antibody 82463-1-RRDownload protocol
IF protocol for VCP antibody 82463-1-RRDownload protocol
IP protocol for VCP antibody 82463-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols