• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

VCP Recombinant antibody, PBS Only

VCP Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G23

Cat no : 82463-1-PBS

Print datasheet

Synonyms

HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit

验证数据展示

  • WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

    WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

    IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VCP antibody included
  • Affinity and Kinetic Characterization of 82463-1-PBS

    Affinity and Kinetic Characterization of 82463-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.

Planning an IHC experiment? We recommend our IHCeasy VCP Ready-To-Use IHC Kit. VCP primary antibody included.

产品信息

82463-1-PBS targets VCP in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen VCP fusion protein Ag1002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name valosin-containing protein
Synonyms HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit
Calculated Molecular Weight 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberBC007562
Gene Symbol VCP
Gene ID (NCBI) 7415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55072
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).