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验证数据展示

  • WB analysis using 82491-1-RR

    WB analysis using 82491-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82491-1-RR (LASP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 82491-1-RR

    IHC staining of human colon using 82491-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82491-1-RR (LASP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LASP1 antibody included
  • IHC staining of human stomach using 82491-1-RR

    IHC staining of human stomach using 82491-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82491-1-RR (LASP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LASP1 antibody included
  • IF Staining of HeLa using 82491-1-RR

    IF Staining of HeLa using 82491-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LASP1 antibody (82491-1-RR, Clone: 4J2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82491-1-RR

    Affinity and Kinetic Characterization of 82491-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82491-1-RR against Human LASP1 were performed. The affinity constant is 10.3 nM.

LASP1 Recombinant antibody

LASP1 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82491-1-RR

产品说明书

CloneNo. 4J2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MLN50, LIM and SH3 protein 1, LASP-1, 4J2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, SKOV-3 cells, PC-3 cells, HCT 116 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82491-1-RR targets LASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LASP1 fusion protein Ag0800 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 LIM and SH3 protein 1
别名 MLN50, LIM and SH3 protein 1, LASP-1, 4J2
计算分子量 30 kDa
观测分子量 35 kDa
GenBank蛋白编号BC012460
基因名称 LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
RRIDAB_3086483
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14847
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

实验方案

Product Specific Protocols
WB protocol for LASP1 antibody 82491-1-RRDownload protocol
IHC protocol for LASP1 antibody 82491-1-RRDownload protocol
IF protocol for LASP1 antibody 82491-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols