LASP1 Recombinant antibody
LASP1 Recombinant Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4J2
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, SKOV-3 cells, PC-3 cells, HCT 116 cells, Jurkat cells, mouse brain tissue, rat brain tissue |
Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82491-1-RR targets LASP1 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, WB, ELISA Application Description |
Tested Reactivity | Human, mouse, rat |
Immunogen | LASP1 fusion protein Ag0800 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | LIM and SH3 protein 1 |
Synonyms | Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50 |
Calculated Molecular Weight | 30 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC012460 |
Gene Symbol | LASP1 |
Gene ID (NCBI) | 3927 |
ENSEMBL Gene ID | ENSG00000002834 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14847 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).
实验方案
Product Specific Protocols | |
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WB protocol for LASP1 antibody 82491-1-RR | Download protocol |
IF protocol for LASP1 antibody 82491-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |