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LASP1 Recombinant antibody, PBS Only

LASP1 Recombinant Antibody for WB,Indirect ELISA,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

4J2

Cat No : 82491-1-PBS

Print datasheet

Synonyms

Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50

验证数据展示

  • WB analysis using 82491-1-RR (same clone as 82491-1-PBS)

    WB analysis using 82491-1-RR (same clone as 82491-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82491-1-RR (LASP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82491-1-RR (same clone as 82491-1-PBS)

    IF Staining of HeLa using 82491-1-RR (same clone as 82491-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LASP1 antibody (82491-1-RR, Clone: 4J2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82491-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.

产品信息

82491-1-PBS targets LASP1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen LASP1 fusion protein Ag0800 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name LIM and SH3 protein 1
Synonyms Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC012460
Gene Symbol LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14847
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).