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WT1 Recombinant antibody

WT1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2J13

Cat no : 82525-1-RR

Print datasheet

Synonyms

Wilms tumor protein, Wilms tumor 1, 2J13

验证数据展示

  • WB analysis using 82525-1-RR

    WB analysis using 82525-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 82525-1-RR

    IP experiment of K-562 using 82525-1-RR

    IP result of anti-WT1 (IP:82525-1-RR, 4ug; Detection:82525-1-RR 1:1000) with K-562 cells lysate 1600 ug.

  • Affinity and Kinetic Characterization of 82525-1-RR

    Affinity and Kinetic Characterization of 82525-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82525-1-RR against Human WT1 were performed. The affinity constant is 0.912 nM.

经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82525-1-RR targets WT1 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen WT1 fusion protein Ag19541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wilms tumor 1
Synonyms Wilms tumor protein, Wilms tumor 1, 2J13
Calculated Molecular Weight 449 aa, 49 kDa, 57 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC032861
Gene Symbol WT1
Gene ID (NCBI) 7490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.

实验方案

Product Specific Protocols
WB protocol for WT1 antibody 82525-1-RRDownload protocol
IP protocol for WT1 antibody 82525-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols