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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82525-1-RR (same clone as 82525-1-PBS)

    WB analysis using 82525-1-RR (same clone as 82525-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82525-1-RR (WT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82525-1-RR (same clone as 82525-1-PBS)

    IHC staining of mouse testis using 82525-1-RR (same clone as 82525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 82525-1-RR (same clone as 82525-1-PBS)

    IP experiment of K-562 using 82525-1-RR (same clone as 82525-1-PBS)

    IP result of anti-WT1 (IP:82525-1-RR, 4ug; Detection:82525-1-RR 1:1000) with K-562 cells lysate 1600 ug. This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 82525-1-RR (same clone as 82525-1-PBS)

    IHC staining of mouse testis using 82525-1-RR (same clone as 82525-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82525-1-RR (WT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82525-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82525-1-RR

    Affinity and Kinetic Characterization of 82525-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82525-1-RR against Human WT1 were performed. The affinity constant is 0.912 nM.

WT1 Recombinant antibody, PBS Only

WT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82525-1-PBS

Print datasheet

CloneNo. 2J13

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Wilms tumor protein, Wilms tumor 1, 2J13

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82525-1-PBS targets WT1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen WT1 fusion protein Ag19541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Wilms tumor 1
Synonyms Wilms tumor protein, Wilms tumor 1, 2J13
Calculated Molecular Weight 449 aa, 49 kDa, 57 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC032861
Gene Symbol WT1
Gene ID (NCBI) 7490
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19544
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.