WT1 Recombinant antibody

WT1 Uni-rAbTM Recombinant Antibody for WB, IP, ELISA
Cat No. 82525-1-RR

产品说明书

CloneNo. 2J13

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

2J13, Wilms tumor 1, Wilms tumor protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82525-1-RR targets WT1 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 WT1 fusion protein Ag19541 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Wilms tumor 1
别名 2J13, Wilms tumor 1, Wilms tumor protein
计算分子量 449 aa, 49 kDa, 57 kDa
观测分子量 52-55 kDa
GenBank蛋白编号BC032861
基因名称 WT1
Gene ID (NCBI) 7490
RRIDAB_3086487
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19544
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for the normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. The function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has a lower affinity for DNA and can bind RNA. WT1 exists some isoforms with a range of molecular weight is 33-50 kDa.

实验方案

Product Specific Protocols
WB protocol for WT1 antibody 82525-1-RRDownload protocol
IP protocol for WT1 antibody 82525-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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