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验证数据展示

  • WB analysis using 82625-4-RR

    WB analysis using 82625-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Ag7791 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82625-4-RR

    WB analysis using 82625-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat dorsal root ganglion using 82625-4-RR

    IHC staining of rat dorsal root ganglion using 82625-4-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 82625-4-RR

    IF Staining of THP-1 using 82625-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Piezo1 (extracellular domain) antibody (82625-4-RR, Clone: 4B15 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82625-4-RR

    Affinity and Kinetic Characterization of 82625-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82625-4-RR against Human Piezo1 (extracellular domain) were performed. The affinity constant is below 1 pM.

Piezo1 (extracellular domain) Recombinant antibody

Piezo1 (extracellular domain) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 82625-4-RR

产品说明书

CloneNo. 4B15

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, hTERT-RPE1 cells
Positive IHC detected inrat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82625-4-RR targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Piezo1 (extracellular domain) fusion protein Ag7791 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 family with sequence similarity 38, member A
别名 FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment
计算分子量 286 kDa
观测分子量 233-286 kDa
GenBank蛋白编号BC008073
基因名称 Piezo1/FAM38A
Gene ID (NCBI) 9780
RRIDAB_3670524
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92508
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

实验方案

Product Specific Protocols
WB protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
IHC protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
IF protocol for Piezo1 (extracellular domain) antibody 82625-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols