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Piezo1 (extracellular domain) Recombinant antibody, PBS Only

Piezo1 (extracellular domain) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B15

Cat no : 82625-4-PBS

Print datasheet

Synonyms

FAM38A, PIEZO1, Piezo-type mechanosensitive ion channel component 1, Membrane protein induced by beta-amyloid treatment, KIAA0233

验证数据展示

  • WB analysis using 82625-4-RR (same clone as 82625-4-PBS)

    WB analysis using 82625-4-RR (same clone as 82625-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Ag7791 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

  • IHC staining of rat dorsal root ganglion using 82625-4-RR (same clone as 82625-4-PBS)

    IHC staining of rat dorsal root ganglion using 82625-4-RR (same clone as 82625-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 82625-4-RR (same clone as 82625-4-PBS)

    IF Staining of THP-1 using 82625-4-RR (same clone as 82625-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Piezo1 (extracellular domain) antibody (82625-4-RR, Clone: 4B15 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82625-4-RR

    Affinity and Kinetic Characterization of 82625-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82625-4-RR against Human Piezo1 (extracellular domain) were performed. The affinity constant is below 1 pM.

产品信息

82625-4-PBS targets Piezo1 (extracellular domain) in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Piezo1 (extracellular domain) fusion protein Ag7791 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name family with sequence similarity 38, member A
Synonyms FAM38A, PIEZO1, Piezo-type mechanosensitive ion channel component 1, Membrane protein induced by beta-amyloid treatment, KIAA0233
Calculated Molecular Weight 286 kDa
Observed Molecular Weight 233-286 kDa
GenBank Accession NumberBC008073
Gene Symbol Piezo1/FAM38A
Gene ID (NCBI) 9780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92508
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)