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CDX2 Recombinant antibody

CDX2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3J1

Cat no : 82659-1-RR

Print datasheet

Synonyms

CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2

验证数据展示

  • WB analysis using 82659-1-RR

    WB analysis using 82659-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse colon using 82659-1-RR

    WB analysis of mouse colon using 82659-1-RR

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 82659-1-RR (CDX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 82659-1-RR

    IHC staining of human colon cancer using 82659-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of human colon cancer using 82659-1-RR

    IHC staining of human colon cancer using 82659-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82659-1-RR (CDX2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IHC staining of rat colon using 82659-1-RR

    IHC staining of rat colon using 82659-1-RR

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82659-1-RR (CDX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDX2 antibody included
  • IF Staining of Caco-2 using 82659-1-RR

    IF Staining of Caco-2 using 82659-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using CDX2 antibody (82659-1-RR, Clone: 3J1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 82659-1-RR

    Affinity and Kinetic Characterization of 82659-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82659-1-RR against Human CDX2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inCaco-2 cells, mouse colon tissue, COLO 320 cells
Positive IHC detected inrat colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy CDX2 Ready-To-Use IHC Kit. CDX2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

82659-1-RR targets CDX2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CDX2 fusion protein Ag17310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name caudal type homeobox 2
Synonyms CDX-2, CDX 3, Caudal-type homeobox protein 2, Caudal type homeobox protein 2, Caudal type homeobox 2
Calculated Molecular Weight 313 aa, 34 kDa
Observed Molecular Weight 33-40 kDa
GenBank Accession NumberBC014461
Gene Symbol CDX2
Gene ID (NCBI) 1045
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99626
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDX2, also named as Homeobox protein CDX-2, is a 313 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the Caudal homeobox family. CDX2 localizes in the nucleus and is involved in the transcriptional regulation of multiple genes expression in the intestinal epithelium. The relative expression of CDX1 to CDX2 protein may be important in the anterior to posterior patterning of the intestinal epithelium and in defining patterns of proliferation and differentiation along the crypt-villus axis. Both Cdx1 and Cdx2 genes must be expressed to reduce tumorigenic potential, to increase sensitivity to apoptosis, and to reduce cell migration, suggesting that the two genes control the normal phenotype by independent pathways.

实验方案

Product Specific Protocols
WB protocol for CDX2 antibody 82659-1-RRDownload protocol
IHC protocol for CDX2 antibody 82659-1-RRDownload protocol
IF protocol for CDX2 antibody 82659-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Biofabrication

3D bioprinting platform development for high-throughput cancer organoid models construction and drug evaluation

Authors - Rui Dai
View Article