Recombinant antibodies

What are recombinant antibodies?

There are many methods of producing recombinant antibodies. At Proteintech, recombinant antibodies are monoclonal antibodies that have been produced by in vitro cloning of the antibody heavy and light chain DNA sequences from the B cells or plasma cells of immunized animals.

Unlike traditional monoclonal antibodies produced from hybridomas, the recombinant vectors are introduced into expression hosts (e.g. E. coli) to then produce the antibodies. This recombinant technology results in almost no lot-to-lot variability, removing the risk of genetic drift that can result in variations in of monoclonal antibodies.

Recombinant antibody production at Proteintech

At Proteintech, we create rabbit recombinant antibodies. Rabbits have superior qualities for recombinant antibody production over other methods, harnessing their natural immune response for its evolutionarily honed epitope recognition, high antibody diversity, and single isotype subclass.

Proteintech’s recombinant antibody development starts with rabbit immunization. As the immune response peaks, antigen-specific single B cells from the rabbits are isolated using our proprietary technology. The selected B cells are then screened for their ability to produce antibodies with highest sensitivity and specificity. The genes for the antibody heavy and light chains from the selected B cells are then cloned into cell lines to express the desired antibody. These antibodies are extensively validated, adhering to the IWGAV (International Working Group on Antibody Validation) recommendation to prove the specificity, sensitivity, and reproducibility.

ChromoTek Recombinant VHH Fusion Antibodies

Nanobodies, the binding domains of heavy chain antibodies from Camelidae family members, can also be fused to Fc-domains to generate chimeric heavy chain antibodies. The Fc-domain fusion format combines the advantages of Nanobodies with those of traditional antibodies: The VHH/Fc fusions (i) are bivalent so they have even higher avidities and affinities than the parent Nanobodies, (ii) bind unique, 3-dimensinal conformations of epitopes not recognized by traditional antibodies, and (iii) can be detected and captured using tools from the broad range of antibody reagents like secondary antibodies and Nano-Secondaries. Like other recombinant antibodies, VHH/Fc-fusions are available with high lot-to-lot consistency at an unlimited supply. The Nanobody Fc-fusion recombinant antibodies have been developed in collaboration with Absolute Antibody Ltd.

Proteintech and ChromoTek offer nanobodies fused to two different Fc-domains - rabbit IgG and mouse IgG1 – for flexibility in your experiments. The popular ChromoTek Nanobodies anti-GFP-, anti-mNeonGreen, anti-TurboGFP-, and anti-vimentin-VHHs are all available in chimeric rabbit IgG and mouse IgG1 form.

Product highlights:

• Popular nanobodies cited in over 2000 papers are fused to rabbit IgG or mouse IgG1 Fc-domains
• Unique epitopes that traditional antibodies do not recognize
• Bivalent structure results in higher avidity and better signal amplification than Nanobodies
• Unlimited supply, high lot-to-lot consistency
• Recombinant expression
• Compatibility with Nano-Secondaries