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  • Featured Product
  • KD/KO Validated

TDP-43 (N-terminal) Recombinant antibody

TDP-43 (N-terminal) Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

11N20

Cat no : 80001-1-RR

Print datasheet

Synonyms

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

验证数据展示

  • WB analysis using 80001-1-RR

    WB analysis using 80001-1-RR

    Various cell lysates were subjected to SDS PAGE followed by western blot with 80001-1-RR (TDP43 antibody) at dilution of 1:35000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 80001-1-RR

    WB analysis of HeLa using 80001-1-RR

    WB result of TDP-43 antibody (80001-1-RR; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 transfected HeLa cells.

  • WB analysis of HAP1 using 80001-1-RR

    WB analysis of HAP1 using 80001-1-RR

    HAP1 (WT and TARDBP KO) lysates prepared with NP-40 buffer, 50 μg protein loaded. 80001-1-RR incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IF Staining of rat brain using 80001-1-RR

    IF Staining of rat brain using 80001-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 80001-1-RR (TDP-43 antibody) at dilution of 1:150 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HAP1 using 80001-1-RR

    IF Staining of HAP1 using 80001-1-RR

    HAP1 WT cells (yellow outline) and TARDBP KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 80001-1-RR at 1:400 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

  • IHC staining of human gliomas using 80001-1-RR

    IHC staining of human gliomas using 80001-1-RR

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of human gliomas using 80001-1-RR

    IHC staining of human gliomas using 80001-1-RR

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of spinal cord slides from ALS patients using 80001-1-RR

    IHC staining of spinal cord slides from ALS patients using 80001-1-RR

    IHC results of TDP43 rabbit recombinant antibody (80001-1-RR, 1:20000) with the spinal cord slides from ALS patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of frontal cortex from FTLD-TDP type B using 80001-1-RR

    IHC staining of frontal cortex from FTLD-TDP type B using 80001-1-RR

    IHC results of TDP43 rabbit recombinant antibody (80001-1-RR, 1:20000) with the frontal cortex from FTLD-TDP type B patients. IHC experiment was done with Ventana automatic staining system and Optiview DAB detection kit with heat-induced epitope retrieval (boiling for 32 min in Tris-EDTA based solution CC1 buffer, Ventana). Fig from the lab of Dr. Neumann.

    Ready-to-use IHC kits available with TDP-43 antibody included
  • IHC staining of rat brain using 80001-1-RR

    IHC staining of rat brain using 80001-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 80001-1-RR (TDP-43 antibody) at dilution of 1:2000 (under 4x lens).

    Ready-to-use IHC kits available with TDP-43 antibody included
  • FC experiment of HeLa using 80001-1-RR

    FC experiment of HeLa using 80001-1-RR

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TDP-43 (80001-1-RR, Clone:11N20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HAP1 cells, K-562 cells, Neuro-2a cells, C6 cells
Positive IHC detected inhuman gliomas tissue, spinal cord slides from ALS patients, frontal cortex from FTLD-TDP type B, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, HAP1 cells
Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TDP-43 Ready-To-Use IHC Kit. TDP-43 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:4000
Immunofluorescence (IF)-PIF-P : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80001-1-RR targets TDP-43 (N-terminal) in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IP, IF
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen fusion protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name TAR DNA binding protein
Synonyms ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC001487
Gene Symbol TDP-43
Gene ID (NCBI) 23435
RRIDAB_2882933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Proteintech's 80001-1-RR is a rabbit recombinant TDP-43 antibody recognizing N-terminal TDP-43. It recognizes the intact 43 kDa protein as well as all posttranslationally modified and truncated forms in multiple applications. Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659, 19823856, 21666678, 22193176) Recently TDP-43 has been reported to be overexpressed in triple negative breast cancer (TNBC) and it may be a potential target for TNBC diagnosis and drug design. (PMID: 29581274).

80001-1-RR can be used in WB, IHC, IHF. For the ICC (IF with cells) experiment, the results will be better with ethanol fixed cell and dilution 1:50 or 1:100. Another antibody with CatNo. 80002-1-RR is recommended for IF or ICC experiment.

实验方案

Product Specific Protocols
WB protocol for TDP-43 (N-terminal) antibody 80001-1-RRDownload protocol
IHC protocol for TDP-43 (N-terminal) antibody 80001-1-RRDownload protocol
IF protocol for TDP-43 (N-terminal) antibody 80001-1-RRDownload protocol
FC protocol for TDP-43 (N-terminal) antibody 80001-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IF

F1000Res

The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence

Authors - Donovan Worrall
View Article