验证数据展示

IF Staining of mouse embryo using 80008-1-RR
E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
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E14.5 FFPE mouse embryo section stained for smooth muscle actin (red, Cat. No 80008-1-RR) and alpha tubulin (green, Cat. CL488-66031). The alpha tubulin was conjugated to CoraLite-488 fluorescent dye. In red, you can see muscle tissue clearly stained including the tongue, heart, and arteries. Nerves and neurons are stained green since they are rich in tubulin. Image credit: @Immunofluorescence on Instagram
WB analysis using 80008-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with smooth muscle actin antibody included
WB analysis using 80008-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 80008-1-RR (a-SMA specific antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with smooth muscle actin antibody included
WB analysis of A549 using 80008-1-RR
WB result of smooth muscle actin specific antibody (80008-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-smooth muscle actin specific transfected A549 cells.
IHC staining of human rectal cancer using 80008-1-RR
Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 80008-1-RR (smooth muscle actin specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with smooth muscle actin antibody included
IF Staining of mouse heart using 80008-1-RR
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using smooth muscle actin specific antibody (80008-1-RR, Clone: 5H7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of C2C12 using 80008-1-RR
1x10^6 C2C12 cells were intracellularly stained with 0.5 ug smooth muscle actin specific Recombinant antibody (80008-1-RR, Clone:5H7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO验证

Alpha smooth muscle actin specific Recombinant antibody

Alpha smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Cat No. 80008-1-RR

产品说明书

CloneNo. 5H7

引用文献(27)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, chicken, zebrafish and More (1)

应用

WB, IHC, IF-P, FC (Intra), ELISA and More (1)

别名

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7

缓冲液配方: PBS and Azide

PBS and Azide

PBS Only

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®594

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (27)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue
Positive IHC detected in	human liver tissue, human colon tissue, mouse colon tissue, rat colon tissue, human heart tissue, human appendicitis tissue, human rectal cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse heart tissue, mouse embryo tissue
Positive FC (Intra) detected in	C2C12 cells

Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:20000-1:100000
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)-P	IF-P : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 20 publications below
IHC	See 10 publications below
IF	See 12 publications below

产品信息

80008-1-RR targets Alpha smooth muscle actin specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, chicken, zebrafish samples.

经测试应用	WB, IHC, IF-P, FC (Intra), ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat, pig, chicken, zebrafish
文献引用反应性	human, mouse, rat, pig, duck
免疫原	Peptide 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Recombinant
产品类型	Antibody
全称	actin, alpha 2, smooth muscle, aorta
别名	ACTA2, Alpha SMA, a-SMA, smooth muscle actin, 5H7
计算分子量	42 kDa
观测分子量	42 kDa
GenBank蛋白编号	NM_001613
基因名称	Alpha smooth muscle actin
Gene ID (NCBI)	59
RRID	AB_2882938
偶联类型	Unconjugated
形式	Liquid
纯化方式	Protein A purification
UNIPROT ID	P62736
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
WB protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol
IHC protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol
IF protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol
FC protocol for Alpha smooth muscle actin specific antibody 80008-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB,IF	Gastroenterology Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells Authors - Decai Wang View Article
mouse	WB,IF	Biomaterials Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure Authors - Lingjun Tong View Article
human	IF	JCI Insight Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease Authors - Peng Xiao View Article
mouse	WB,IHC	Pharmacol Res MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2. Authors - Yue Zhang View Article
human,mouse	WB,IHC,IF	Int Immunopharmacol TSLP/TSLPR promotes renal fibrosis by activating STAT3 in renal fibroblasts Authors - Decai Wang View Article
mouse	WB	Eur J Pharmacol Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway Authors - Shaoling Lin View Article

验证数据展示

IF Staining of mouse embryo using 80008-1-RR

WB analysis using 80008-1-RR

IHC staining of human liver using 80008-1-RR

WB analysis using 80008-1-RR

IHC staining of human colon using 80008-1-RR

WB analysis of A549 using 80008-1-RR

IHC staining of human rectal cancer using 80008-1-RR

IF Staining of mouse heart using 80008-1-RR

FC experiment of C2C12 using 80008-1-RR

Alpha smooth muscle actin specific Recombinant antibody

Alpha smooth muscle actin specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Pancreatic acinar cells-derived sphingosine-1-phosphate contributes to fibrosis of chronic pancreatitis via inducing autophagy and activation of pancreatic stellate cells

Myocardial delivery of miR30d with peptide-functionalized milk-derived extracellular vesicles for targeted treatment of hypertrophic heart failure

Targeting hyaluronan synthesis enhances the therapeutic effectiveness of biologics in inflammatory bowel disease

MicroRNA-24-3p alleviates cardiac fibrosis by suppressing cardiac fibroblasts mitophagy via downregulating PHB2.

TSLP/TSLPR promotes renal fibrosis by activating STAT3 in renal fibroblasts

Palmatine alleviates cardiac fibrosis by inhibiting fibroblast activation through the STAT3 pathway

Alpha smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, FC (Intra), ELISA