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验证数据展示

  • FC experiment of C2C12 using CL488-80008

    FC experiment of C2C12 using CL488-80008

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human smooth muscle actin specific (CL488-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse heart using CL488-80008

    IF Staining of mouse heart using CL488-80008

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin specific antibody (CL488-80008, Clone: 5H7 ) at dilution of 1:200.

  • IF Staining of mouse heart using CL488-80008

    IF Staining of mouse heart using CL488-80008

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin specific antibody (CL488-80008, Clone: 5H7 ) at dilution of 1:200.

  • IF Staining of H9C2 using CL488-80008

    IF Staining of H9C2 using CL488-80008

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite® Plus 488 smooth muscle actin specific antibody (CL488-80008, Clone: 5H7 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Alpha smooth muscle actin specific Recombinant antibody

Alpha smooth muscle actin specific Recombinant Antibody for IF/ICC, IF-P, FC (Intra)
Cat No. CL488-80008

产品说明书

CloneNo. 5H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, chicken

应用

IF/ICC, IF-P, FC (Intra)

别名

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80008-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80008 targets Alpha smooth muscle actin specific in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

经测试应用 IF/ICC, IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig, chicken
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, alpha 2, smooth muscle, aorta
别名 ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form
计算分子量 42 kDa
观测分子量42 kDa
GenBank蛋白编号NM_001613
基因名称 Alpha smooth muscle actin
Gene ID (NCBI) 59
RRIDAB_2923883
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP62736
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha smooth muscle actin specific antibody CL488-80008Download protocol
FC protocol for CL Plus 488 Alpha smooth muscle actin specific antibody CL488-80008Download protocol
Standard Protocols
Click here to view our Standard Protocols