- Featured Product
- KD/KO Validated
CoraLite®594-conjugated Alpha smooth muscle actin specific Recombinant antibody
Alpha smooth muscle actin specific Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig, chicken
Applications
IF/ICC, IF-P, FC (Intra) and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
5H7
验证数据展示
经过测试的应用
Positive IF-P detected in | mouse heart tissue |
Positive IF/ICC detected in | H9C2 cells |
Positive FC (Intra) detected in | C2C12 cells |
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
CL594-80008 targets Alpha smooth muscle actin specific in WB, IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.
Tested Applications | IF/ICC, IF-P, FC (Intra) Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat, pig, chicken |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | actin, alpha 2, smooth muscle, aorta |
Synonyms | ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form |
Calculated Molecular Weight | 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | NM_001613 |
Gene Symbol | Alpha smooth muscle actin |
Gene ID (NCBI) | 59 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P62736 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 | Download protocol |
FC protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |