ChromoTek GFP RecAb, VHH-mouse IgG1 Fc fusion
Mouse monoclonal antibody to GFP. Recombinant fusion of anti-GFP VHH [CTK0201] and mouse IgG1 Fc domain.
Host/IsoType
Mouse/IgG1
Specificity
GFP and GFP derivates
Applications
IF
Conjugate
Unconjugated
Clone No.
CTK0201
Cat no : gfms
Synonyms
Validation Data Gallery
![HeLa cells transiently expressing Mannosidase II-EGFP were immunostained with GFP recombinant antibody, VHH-mouse IgG1 Fc fusion [CTK0201] (gfms, 1:2,000) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647 [CTK0103, CTK0104] (sms1AF647-1, 1:500). Scale bar, 10 μM.](/products/pictures/gfms-1.jpg "HeLa cells transiently expressing Mannosidase II-EGFP were immunostained with GFP recombinant antibody, VHH-mouse IgG1 Fc fusion [CTK0201] (gfms, 1:2,000) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647 [CTK0103, CTK0104] (sms1AF647-1, 1:500). Scale bar, 10 μM.")
Product Information
Mouse monoclonal antibody to GFP. Recombinant fusion of anti-GFP VHH [CTK0201] and mouse IgG1 Fc domain.
| Applications | IF |
| Host/IsoType | Mouse/IgG1 |
| Species Specificity | Recombinant protein from Aequorea victoria |
| Specificity/Target | Green fluorescent protein (GFP) and derivates thereof such as Citrine, CFP, EGFP, EYFP, TagGFP, Venus, YFP |
| Conjugate | Unconjugated |
| Recommended Dilution | IF/ICC: 1:400 - 1:2,000 |
| GenBank Accession Number | U17997 |
| Purification Method | Recombinantly expressed and purified by IMAC |
| Type | Primary antibody; chimeric fusion of Nanobody (VHH) and rabbit Fc domain |
| Class | Recombinant |
| Format | Recombinant monoclonal mouse antibody |
| Immunogen | Purified recombinant GFP [Aequorea victoria] |
| Clonality | Monoclonal |
| Clone No. | CTK0201 |
| Calculated MW | 78.9 kDa |
| Form | Buffered aqueous solution |
| RRID | AB_2889366 |
| Storage Buffer | PBS, 10% glycerol, Preservative: 0.02 % sodium azide, safety datasheet (SDS): sodium azide |
| Storage Condition | Shipped on ice. Aliquot upon receipt and store at -20°C/-4°F. Avoid freeze-thaw cycles. |
| Size | 20 µL; 100 µL |
Publications
| Application | Title |
|---|---|
Proc Natl Acad Sci U S A Robust designation of meiotic crossover sites by CDK-2 through phosphorylation of the MutSγ complex. |