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验证数据展示

  • WB analysis using 82666-14-RR

    WB analysis using 82666-14-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82666-14-RR (VDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293T using 82666-14-RR

    WB analysis of HEK-293T using 82666-14-RR

    WB result of VDAC3 antibody (82666-14-RR; 1:4000; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells.

  • IHC staining of human ovary cancer using 82666-14-RR

    IHC staining of human ovary cancer using 82666-14-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82666-14-RR (VDAC3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

VDAC3 Recombinant antibody

VDAC3 Recombinant Antibody for WB, IHC, ELISA
Cat No. 82666-14-RR

产品说明书

CloneNo. 230451C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HEK-293T cells, NIH/3T3 cells, HeLa cells, A431 cells, mouse heart tissue, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82666-14-RR targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 voltage-dependent anion channel 3
别名 VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7
计算分子量 31 kDa
观测分子量 31 kDa
GenBank蛋白编号NM_005662
基因名称 VDAC3
Gene ID (NCBI) 7419
RRIDAB_3670525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y277
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

实验方案

Product Specific Protocols
WB protocol for VDAC3 antibody 82666-14-RRDownload protocol
IHC protocol for VDAC3 antibody 82666-14-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols