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CoraLite® Plus 750-conjugated VDAC3 Recombinant antibody

VDAC3 Uni-rAbTM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230451C7

Cat no : CL750-82666-14

Print datasheet

Synonyms

VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7

验证数据展示

  • WB analysis using CL750-82666-14

    WB analysis using CL750-82666-14

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82666-14 (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using CL750-82666-14

    FC experiment of HeLa using CL750-82666-14

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated VDAC3 Recombinant antibody (CL750-82666-14, Clone:230451C7)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, HeLa cells，
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 82666-14-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82666-14 targets VDAC3 in WB applications and shows reactivity with human, mouse samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name voltage-dependent anion channel 3
Synonyms VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberNM_005662
Gene Symbol VDAC3
Gene ID (NCBI) 7419
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y277
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 VDAC3 antibody CL750-82666-14Download protocol
Standard Protocols
Click here to view our Standard Protocols