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MFN2 Recombinant antibody

MFN2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5

Cat no : 82673-2-RR

Print datasheet

Synonyms

MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1

验证数据展示

  • WB analysis using 82673-2-RR

    WB analysis using 82673-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82673-2-RR (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82673-2-RR

    IF Staining of HepG2 using 82673-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MFN2 antibody (82673-2-RR, Clone: 2A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon cancer using 82673-2-RR

    IHC staining of human colon cancer using 82673-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MFN2 antibody included
  • IHC staining of human colon cancer using 82673-2-RR

    IHC staining of human colon cancer using 82673-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MFN2 antibody included
  • Affinity and Kinetic Characterization of 82673-2-RR

    Affinity and Kinetic Characterization of 82673-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82673-2-RR against Human MFN2 were performed. The affinity constant is 19.7 nM.

经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MFN2 Ready-To-Use IHC Kit. MFN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82673-2-RR targets MFN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen MFN2 fusion protein Ag2845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitofusin 2
Synonyms MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1
Calculated Molecular Weight 757 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC017061
Gene Symbol MFN2
Gene ID (NCBI) 9927
ENSEMBL Gene IDENSG00000116688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95140
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).

实验方案

Product Specific Protocols
WB protocol for MFN2 antibody 82673-2-RRDownload protocol
IHC protocol for MFN2 antibody 82673-2-RRDownload protocol
IF protocol for MFN2 antibody 82673-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols