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MFN2 Recombinant antibody, PBS Only

MFN2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5

Cat no : 82673-2-PBS

Print datasheet

Synonyms

MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1

验证数据展示

  • WB analysis using 82673-2-RR (same clone as 82673-2-PBS)

    WB analysis using 82673-2-RR (same clone as 82673-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82673-2-RR (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82673-2-RR (same clone as 82673-2-PBS)

    IF Staining of HepG2 using 82673-2-RR (same clone as 82673-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MFN2 antibody (82673-2-RR, Clone: 2A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN2 antibody included
  • IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN2 antibody included
  • Affinity and Kinetic Characterization of 82673-2-RR

    Affinity and Kinetic Characterization of 82673-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82673-2-RR against Human MFN2 were performed. The affinity constant is 19.7 nM.

Planning an IHC experiment? We recommend our IHCeasy MFN2 Ready-To-Use IHC Kit. MFN2 primary antibody included.

产品信息

82673-2-PBS targets MFN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen MFN2 fusion protein Ag2845 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mitofusin 2
Synonyms MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1
Calculated Molecular Weight 757 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC017061
Gene Symbol MFN2
Gene ID (NCBI) 9927
ENSEMBL Gene IDENSG00000116688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95140
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).