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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82673-2-RR (same clone as 82673-2-PBS)

    WB analysis using 82673-2-RR (same clone as 82673-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82673-2-RR (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82673-2-RR (same clone as 82673-2-PBS)

    IF Staining of HepG2 using 82673-2-RR (same clone as 82673-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MFN2 antibody (82673-2-RR, Clone: 2A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN2 antibody included
  • IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    IHC staining of human colon cancer using 82673-2-RR (same clone as 82673-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82673-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MFN2 antibody included
  • Affinity and Kinetic Characterization of 82673-2-RR

    Affinity and Kinetic Characterization of 82673-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82673-2-RR against Human MFN2 were performed. The affinity constant is 19.7 nM.

MFN2 Recombinant antibody, PBS Only

MFN2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82673-2-PBS

产品说明书

CloneNo. 2A5

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy MFN2 Ready-To-Use IHC Kit. MFN2 primary antibody included.

产品信息

82673-2-PBS targets MFN2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 MFN2 fusion protein Ag2845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 mitofusin 2
别名 MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1
计算分子量 757 aa, 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC017061
基因名称 MFN2
Gene ID (NCBI) 9927
ENSEMBL Gene IDENSG00000116688
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95140
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).