PD-L1/CD274 Recombinant antibody

PD-L1/CD274 Uni-rAbTM Recombinant Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G4

Cat no : 82719-13-RR

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Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1



经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82719-13-RR targets PD-L1/CD274 in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen PD-L1/CD274 fusion protein Ag12432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD274 molecule
Synonyms CD274, PD-L1, PD L1, hPD-L1, B7-H1
Calculated Molecular Weight 290 aa, 33 kDa
GenBank Accession NumberBC074984
Gene Symbol PD-L1
Gene ID (NCBI) 29126
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZQ7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, it is predicted to be 27-30 kDa after signal peptide cleavage (PMID: 25609200; 17076679). The apparent molecular weight has also been reported as 45-70 kDa, major glycosylated form of 45-50 kDa and multiple post-translational modifications form of 65-70 kDa (PMID: 18760278; 16493058).

实验方案

Product Specific Protocols
IHC protocol for PD-L1/CD274 antibody 82719-13-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols