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TTL Recombinant antibody

TTL Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3P9

Cat no : 82763-5-RR

Print datasheet

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9

验证数据展示

  • WB analysis using 82763-5-RR

    WB analysis using 82763-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82763-5-RR (TTL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82763-5-RR

    WB analysis of HeLa using 82763-5-RR

    WB result of TTL antibody (82763-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells.

  • Affinity and Kinetic Characterization of 82763-5-RR

    Affinity and Kinetic Characterization of 82763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82763-5-RR against Human TTL were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, SH-SY5Y cells, U2OS cells, Y79 cells, Jurkat cells, mouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82763-5-RR targets TTL in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen TTL fusion protein Ag4526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tubulin tyrosine ligase
Synonyms Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC036819
Gene Symbol TTL
Gene ID (NCBI) 150465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NG68
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.

实验方案

Product Specific Protocols
WB protocol for TTL antibody 82763-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols