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  • Featured Product
  • KD/KO Validated

TTL Recombinant antibody, PBS Only

TTL Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3P9

Cat no : 82763-5-PBS

Print datasheet

Synonyms

Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9

验证数据展示

  • WB analysis using 82763-5-RR (same clone as 82763-5-PBS)

    WB analysis using 82763-5-RR (same clone as 82763-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82763-5-RR (TTL antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82763-5-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82763-5-RR (same clone as 82763-5-PBS)

    WB analysis of HeLa using 82763-5-RR (same clone as 82763-5-PBS)

    WB result of TTL antibody (82763-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTL transfected HeLa cells. This data was developed using the same antibody clone with 82763-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82763-5-RR

    Affinity and Kinetic Characterization of 82763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82763-5-RR against Human TTL were performed. The affinity constant is below 1 pM.

产品信息

82763-5-PBS targets TTL in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TTL fusion protein Ag4526 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tubulin tyrosine ligase
Synonyms Tubulin--tyrosine ligase, tubulin tyrosine ligase, Tubulin tyrosine ligase, EC:6.3.2.25, 3P9
Calculated Molecular Weight 377 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC036819
Gene Symbol TTL
Gene ID (NCBI) 150465
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NG68
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tubulin-tyrosine ligase (TTL) is the enzyme responsible for the reversible addition of a tyrosine residue at the carboxyl end of alpha-tubulin. TTL forms stable complexes with tubulin and inhibit tubulin polymerization. TTL is frequently suppressed during tumor progression with resulting accumulation of detyrosinated alpha-tubulin in tumor cells. TTL suppression in human cancers is associated with increased tumor aggressiveness.