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验证数据展示

  • WB analysis using 82765-1-RR

    WB analysis using 82765-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 82765-1-RR

    IHC staining of human stomach using 82765-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82765-1-RR (Thioredoxin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TXN antibody included
  • Affinity and Kinetic Characterization of 82765-1-RR

    Affinity and Kinetic Characterization of 82765-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82765-1-RR against Human Thioredoxin were performed. The affinity constant is 0.437 nM.

Thioredoxin Recombinant antibody

Thioredoxin Recombinant Antibody for WB, IHC, ELISA
Cat No. 82765-1-RR

产品说明书

CloneNo. 7G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

TXN, THIO, Surface-associated sulphydryl protein, Hom s Trx, ATL-derived factor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TXN Ready-To-Use IHC Kit. TXN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82765-1-RR targets Thioredoxin in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Thioredoxin fusion protein Ag6989 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 thioredoxin
别名 TXN, THIO, Surface-associated sulphydryl protein, Hom s Trx, ATL-derived factor
计算分子量 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC003377
基因名称 TXN
Gene ID (NCBI) 7295
RRIDAB_3086531
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10599
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

实验方案

Product Specific Protocols
WB protocol for Thioredoxin antibody 82765-1-RRDownload protocol
IHC protocol for Thioredoxin antibody 82765-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols