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Thioredoxin Recombinant antibody, PBS Only

Thioredoxin Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7G10

Cat no : 82765-1-PBS

Print datasheet

Synonyms

TXN, THIO, Surface-associated sulphydryl protein, Hom s Trx, ATL-derived factor

验证数据展示

  • WB analysis using 82765-1-RR (same clone as 82765-1-PBS)

    WB analysis using 82765-1-RR (same clone as 82765-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82765-1-RR (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82765-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 82765-1-RR (same clone as 82765-1-PBS)

    IHC staining of human stomach using 82765-1-RR (same clone as 82765-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82765-1-RR (Thioredoxin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82765-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TXN antibody included
  • Affinity and Kinetic Characterization of 82765-1-RR

    Affinity and Kinetic Characterization of 82765-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82765-1-RR against Human Thioredoxin were performed. The affinity constant is 0.437 nM.

Planning an IHC experiment? We recommend our IHCeasy TXN Ready-To-Use IHC Kit. TXN primary antibody included.

产品信息

82765-1-PBS targets Thioredoxin in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Thioredoxin fusion protein Ag6989 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name thioredoxin
Synonyms TXN, THIO, Surface-associated sulphydryl protein, Hom s Trx, ATL-derived factor
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC003377
Gene Symbol TXN
Gene ID (NCBI) 7295
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10599
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.