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OGDH Recombinant antibody

OGDH Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C16

Cat No : 82773-1-RR

Print datasheet

Synonyms

AKGDH, E1k, OGDC, OGDC E1, OGDH

验证数据展示

  • WB analysis using 82773-1-RR

    WB analysis using 82773-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue, A549 cells, NIH/3T3 cells, C6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82773-1-RR targets OGDH in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen OGDH fusion protein Ag7368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms AKGDH, E1k, OGDC, OGDC E1, OGDH
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

实验方案

Product Specific Protocols
WB protocol for OGDH antibody 82773-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols