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OGDH Recombinant antibody, PBS Only

OGDH Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C16

Cat no : 82773-1-PBS

Print datasheet

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

验证数据展示

  • WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

    WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with OGDH antibody included
Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.

产品信息

82773-1-PBS targets OGDH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OGDH fusion protein Ag7368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.