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OGDH Recombinant antibody

OGDH Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C16

Cat no : 82773-1-RR

Print datasheet

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

验证数据展示

  • WB analysis using 82773-1-RR

    WB analysis using 82773-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with OGDH antibody included
  • IF Staining of mouse kidney using 82773-1-RR

    IF Staining of mouse kidney using 82773-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using OGDH antibody (82773-1-RR, Clone: 1C16 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue, A549 cells, NIH/3T3 cells, C6 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy OGDH Ready-To-Use IHC Kit. OGDH primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82773-1-RR targets OGDH in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen OGDH fusion protein Ag7368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Synonyms E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberBC004964
Gene Symbol OGDH
Gene ID (NCBI) 4967
RRIDAB_3670547
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02218
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.

实验方案

Product Specific Protocols
WB protocol for OGDH antibody 82773-1-RRDownload protocol
IHC protocol for OGDH antibody 82773-1-RRDownload protocol
IF protocol for OGDH antibody 82773-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols