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MSH2 Recombinant antibody

MSH2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6O4

Cat no : 82775-1-RR

Print datasheet

Synonyms

MSH 2, 6O4

验证数据展示

  • WB analysis using 82775-1-RR

    WB analysis using 82775-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82775-1-RR (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 82775-1-RR

    IHC staining of human colon cancer using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 82775-1-RR (MSH2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human tonsillitis using 82775-1-RR

    IHC staining of human tonsillitis using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82775-1-RR (MSH2 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included
  • IHC staining of human colon using 82775-1-RR

    IHC staining of human colon using 82775-1-RR

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82775-1-RR (MSH2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MSH2 antibody included
  • Affinity and Kinetic Characterization of 82775-1-RR

    Affinity and Kinetic Characterization of 82775-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82775-1-RR against Human MSH2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Caco-2 cells, A549 cells, HCT 116 cells
Positive IHC detected inhuman colon tissue, human colon cancer, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MSH2 Ready-To-Use IHC Kit. MSH2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82775-1-RR targets MSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen MSH2 fusion protein Ag7835 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
Synonyms MSH 2, 6O4
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 100 kDa-105 kDa
GenBank Accession NumberBC021566
Gene Symbol MSH2
Gene ID (NCBI) 4436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43246
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

实验方案

Product Specific Protocols
WB protocol for MSH2 antibody 82775-1-RRDownload protocol
IHC protocol for MSH2 antibody 82775-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols