RACGAP1 Recombinant antibody
RACGAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2D5
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, U2OS cells, K-562 cells, Jurkat cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82788-1-RR targets RACGAP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
Tested Reactivity | human |
Immunogen | RACGAP1 fusion protein Ag4676 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | Rac GTPase activating protein 1 |
Synonyms | HsCYK4, HsCYK 4, Gap1, GAP, CYK4 |
Calculated Molecular Weight | 632 aa, 71 kDa |
Observed Molecular Weight | 71 kDa |
GenBank Accession Number | BC032754 |
Gene Symbol | RACGAP1 |
Gene ID (NCBI) | 29127 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9H0H5 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RACGAP1 antibody 82788-1-RR | Download protocol |
IHC protocol for RACGAP1 antibody 82788-1-RR | Download protocol |
IF protocol for RACGAP1 antibody 82788-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |