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UPF1 Recombinant antibody

UPF1 Recombinant Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3O15

Cat No : 82791-1-RR

Print datasheet

Synonyms

ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1

验证数据展示

  • WB analysis using 82791-1-RR

    WB analysis using 82791-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82791-1-RR

    IF Staining of HepG2 using 82791-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

经过测试的应用

Positive WB detected inrat testis tissue, Raji cells, SH-SY5Y cells, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:185-1:740
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82791-1-RR targets UPF1 in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human, rat
Immunogen UPF1 fusion protein Ag29057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms ATP dependent helicase RENT1, FLJ43809, FLJ46894, HUPF1, KIAA0221, NORF1, pNORF1, RENT1, UPF1
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

实验方案

Product Specific Protocols
WB protocol for UPF1 antibody 82791-1-RRDownload protocol
IF protocol for UPF1 antibody 82791-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols