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UPF1 Recombinant antibody, PBS Only

UPF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O15

Cat no : 82791-1-PBS

Print datasheet

Synonyms

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

验证数据展示

  • WB analysis using 82791-1-RR (same clone as 82791-1-PBS)

    WB analysis using 82791-1-RR (same clone as 82791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82791-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82791-1-RR (same clone as 82791-1-PBS)

    IF Staining of HepG2 using 82791-1-RR (same clone as 82791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82791-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82791-1-RR

    Affinity and Kinetic Characterization of 82791-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82791-1-RR against Human UPF1 were performed. The affinity constant is 50.7 nM.

产品信息

82791-1-PBS targets UPF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity rat, human
Immunogen UPF1 fusion protein Ag29057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92900
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.