验证数据展示
经过测试的应用
Positive WB detected in | rat testis tissue, Raji cells, SH-SY5Y cells, HEK-293 cells, HepG2 cells, HeLa cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:185-1:740 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82791-1-RR targets UPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | human, rat |
免疫原 | UPF1 fusion protein Ag29057 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | UPF1 regulator of nonsense transcripts homolog (yeast) |
别名 | RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1 |
计算分子量 | 123 kDa |
观测分子量 | 123-130 kDa |
GenBank蛋白编号 | BC039817 |
基因名称 | UPF1 |
Gene ID (NCBI) | 5976 |
RRID | AB_3086538 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q92900 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for UPF1 antibody 82791-1-RR | Download protocol |
IF protocol for UPF1 antibody 82791-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |