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验证数据展示

  • WB analysis using 82791-1-RR

    WB analysis using 82791-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82791-1-RR (UPF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82791-1-RR

    IF Staining of HepG2 using 82791-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UPF1 antibody (82791-1-RR, Clone: 3O15 ) at dilution of 1:370 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82791-1-RR

    Affinity and Kinetic Characterization of 82791-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82791-1-RR against Human UPF1 were performed. The affinity constant is 50.7 nM.

UPF1 Recombinant antibody

UPF1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82791-1-RR

Print datasheet

CloneNo. 3O15

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat testis tissue, Raji cells, SH-SY5Y cells, HEK-293 cells, HepG2 cells, HeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:185-1:740
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82791-1-RR targets UPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, rat
Immunogen UPF1 fusion protein Ag29057 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name UPF1 regulator of nonsense transcripts homolog (yeast)
Synonyms RENT1, Regulator of nonsense transcripts 1, KIAA0221, EC:3.6.4.12, ATP-dependent helicase RENT1
Calculated Molecular Weight123 kDa
Observed Molecular Weight 123-130 kDa
GenBank Accession NumberBC039817
Gene Symbol UPF1
Gene ID (NCBI) 5976
RRIDAB_3086538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Up-Frameshift Suppressor 1 Homolog (UPF1) is the central factor in nonsense-mediated mRNA decay (NMD) and is also directly involved in telomere homeostasis, DNA replication, histone mRNA degradation and staufen-mediated mRNA decay (PMID: 29382845). It is a potential modulator of MALAT1 and that UPF1/MALAT1 pathway could be a therapeutic target for gastric cancer (PMID: 28942451). The molecular mass of UPF1 is 123-130 kDa.

实验方案

Product Specific Protocols
WB protocol for UPF1 antibody 82791-1-RRDownload protocol
IF protocol for UPF1 antibody 82791-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols