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EDNRB Recombinant antibody

EDNRB Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1O2

Cat No : 82818-2-RR

Print datasheet

Synonyms

ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor

验证数据展示

  • WB analysis using 82818-2-RR

    WB analysis using 82818-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82818-2-RR (EDNRB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • FC experiment of Raji using 82818-2-RR

    FC experiment of Raji using 82818-2-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug EDNRB Recombinant antibody (82818-2-RR, Clone:1O2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive FC (Intra) detected inRaji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82818-2-RR targets EDNRB in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name endothelin receptor type B
Synonyms ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor
Calculated Molecular Weight 50 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_000115
Gene Symbol EDNRB
Gene ID (NCBI) 1910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).

实验方案

Product Specific Protocols
WB protocol for EDNRB antibody 82818-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols