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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82818-2-RR (same clone as 82818-2-PBS)

    WB analysis using 82818-2-RR (same clone as 82818-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82818-2-RR (EDNRB antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82818-2-PBS in a different storage buffer formulation.

  • FC experiment of Raji using 82818-2-RR (same clone as 82818-2-PBS)

    FC experiment of Raji using 82818-2-RR (same clone as 82818-2-PBS)

    1x10^6 Raji cells were intracellularly stained with 0.25 ug EDNRB Recombinant antibody (82818-2-RR, Clone:1O2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82818-2-PBS in a different storage buffer formulation.

EDNRB Recombinant antibody, PBS Only

EDNRB Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 82818-2-PBS

产品说明书

CloneNo. 1O2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82818-2-PBS targets EDNRB in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 endothelin receptor type B
别名 ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor
计算分子量 50 kDa
观测分子量60 kDa
GenBank蛋白编号NM_000115
基因名称 EDNRB
Gene ID (NCBI) 1910
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24530
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).