验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue |
| Positive FC (Intra) detected in | Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82818-2-RR targets EDNRB in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | endothelin receptor type B |
| 别名 | ET BR, ET B, endothelin receptor type B, Endothelin receptor non-selective type, Endothelin B receptor |
| 计算分子量 | 50 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | NM_000115 |
| 基因名称 | EDNRB |
| Gene ID (NCBI) | 1910 |
| RRID | AB_3670561 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P24530 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EDNRB (endothelin receptor type B) is a G-protein coupled heptahelical endothelin-1 (EDN1) receptor. EDN1, a known mediator of human myometrial contraction in vitro, belongs to a family of three 21-amino acid isopeptides (PMID:18497345). EDNRB is a 442-residue protein expressed post-natally in many tissues, particularly in the myenteric plexus, mucosal layer, ganglia, and blood vessels of the submucosa of the colon (PMID:11434563). Moreover, Both the EDNRA and EDNRB are expressed in vessels and can be bound by EDN1, but while EDNRA mediates a constrictive effect, EDNRB is involved in vasorelaxation and functions as a clearance receptor that scavenges circulating EDN1 (PMID:30171849).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EDNRB antibody 82818-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |