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FBXO44 Recombinant antibody

FBXO44 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230229G7

Cat no : 82857-2-RR

Print datasheet

Synonyms

FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44

验证数据展示

  • WB analysis using 82857-2-RR

    WB analysis using 82857-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82857-2-RR (FBXO44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82857-2-RR

    IHC staining of mouse brain using 82857-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82857-2-RR

    IHC staining of mouse brain using 82857-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82857-2-RR (FBXO44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 82857-2-RR

    IF Staining of A431 using 82857-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of A431 using 82857-2-RR

    FC experiment of A431 using 82857-2-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug FBXO44 Recombinant antibody (82857-2-RR, Clone:230229G7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of U2OS using 82857-2-RR

    IF Staining of U2OS using 82857-2-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXO44 antibody (82857-2-RR, Clone: 230229G7 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82857-2-RR

    Affinity and Kinetic Characterization of 82857-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82857-2-RR against Human FBXO44 were performed. The affinity constant is 0.134 nM.

经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells, A549 cells, HeLa cells, HEK-293 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells, U2OS cells
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82857-2-RR targets FBXO44 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen FBXO44 fusion protein Ag0977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name F-box protein 44
Synonyms FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44
Calculated Molecular Weight 26 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC007832
Gene Symbol FBXO44
Gene ID (NCBI) 93611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4M3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

实验方案

Product Specific Protocols
WB protocol for FBXO44 antibody 82857-2-RRDownload protocol
IHC protocol for FBXO44 antibody 82857-2-RRDownload protocol
IF protocol for FBXO44 antibody 82857-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols