验证数据展示
经过测试的应用
Positive IF/ICC detected in | A431 cells |
Positive FC (Intra) detected in | A431 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-82857-2 targets FBXO44 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human, mouse |
免疫原 | FBXO44 fusion protein Ag0977 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | F-box protein 44 |
别名 | FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44 |
计算分子量 | 26 kDa |
观测分子量 | 30 kDa |
GenBank蛋白编号 | BC007832 |
基因名称 | FBXO44 |
Gene ID (NCBI) | 93611 |
RRID | AB_3673086 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9H4M3 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 FBXO44 antibody CL488-82857-2 | Download protocol |
FC protocol for CL Plus 488 FBXO44 antibody CL488-82857-2 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |