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验证数据展示

  • IF Staining of A431 using CL488-82857-2

    IF Staining of A431 using CL488-82857-2

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 FBXO44 antibody (CL488-82857-2, Clone: 230229G7 ) at dilution of 1:200.

  • FC experiment of A431 using CL488-82857-2

    FC experiment of A431 using CL488-82857-2

    1x10^6 A431 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Fbxo44 Recombinant Antibody (CL488-82857-2, Clone:230229G7)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated FBXO44 Recombinant antibody

FBXO44 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82857-2

Print datasheet

CloneNo. 230229G7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 82857-2-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82857-2 targets FBXO44 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen FBXO44 fusion protein Ag0977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name F-box protein 44
Synonyms FBG3, F box/G domain protein 3, F box protein FBX30, F box protein 44, F box only protein 44
Calculated Molecular Weight 26 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC007832
Gene Symbol FBXO44
Gene ID (NCBI) 93611
RRIDAB_3673086
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H4M3
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXO44 is a member of the F-box protein family that shares a function as substrate recognition factors for the SKP1-CUL1-F-box (SCF)-type ubiquitin ligase. Several human FBXs (FBXO2, FBXO6, FBXO17, FBXO27, and FBXO44) share a conserved G domain (also known as FBA domain), which is responsible for recognizing N-glycan moieties by most FBXs in this group except FBXO44. (PMID: 25970626, PMID: 23086937)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 FBXO44 antibody CL488-82857-2Download protocol
FC protocol for CL Plus 488 FBXO44 antibody CL488-82857-2Download protocol
Standard Protocols
Click here to view our Standard Protocols