NOX2 Recombinant antibody
NOX2 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF-P, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1B17
验证数据展示
经过测试的应用
| Positive WB detected in | mouse spleen tissue, rat spleen tissue |
| Positive IF-P detected in | mouse spleen tissue |
| Positive FC (Intra) detected in | RAW 264.7 cells |
Planning an IHC experiment? We recommend our IHCeasy CYBB/NOX2 Ready-To-Use IHC Kit. CYBB/NOX2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:1000-1:4000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82872-1-RR targets NOX2 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF-P, FC (Intra), ELISA Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | NOX2 fusion protein Ag4402 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | cytochrome b-245, beta polypeptide |
| Synonyms | CYBB, EC:1.-.-.-, Cytochrome b-245 heavy chain, CGD91-phox, CGD |
| Calculated Molecular Weight | 577 aa, 65 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC032720 |
| Gene Symbol | NOX2 |
| Gene ID (NCBI) | 1536 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04839 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NOX2, also named as CYBB, CGD, 91-phox, gp91-1, gp91-phox, p22 phagocyte B-cytochrome, cytochrome b-245 and beta polypeptide, is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. This full length protein has three glycosylation sites. CYBB is found in human cardiomyocytes as multiple bands:the signal between 55 and 65 kDa is probably the unglycosylated protein, because the predicted molecular weight of unglycosylated CYBB protein is approximately 58 kDa(PMID: 17587483) to 65 kDa in phagocytes and the bands around 80 kDa probably represent glycosylated CYBB, as has also been shown in human umbilical vein endothelial cells(PMID:12610097). In other reports, it also can be detected a band of 91 kDa(PMID:19965781).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NOX2 antibody 82872-1-RR | Download protocol |
| IF protocol for NOX2 antibody 82872-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
