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  • Featured Product
  • KD/KO Validated

DEK Recombinant antibody

DEK Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230065H2

Cat no : 82880-1-RR

Print datasheet

Synonyms

D6S231E, 230065H2

验证数据展示

  • WB analysis using 82880-1-RR

    WB analysis using 82880-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82880-1-RR (DEK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82880-1-RR

    WB analysis of HEK-293 using 82880-1-RR

    WB result of DEK antibody (82880-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DEK transfected HEK-293 cells.

  • IF Staining of U2OS using 82880-1-RR

    IF Staining of U2OS using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • IF Staining of HeLa using 82880-1-RR

    IF Staining of HeLa using 82880-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (82880-1-RR, Clone: 230065H2 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • FC experiment of HeLa using 82880-1-RR

    FC experiment of HeLa using 82880-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DEK Recombinant antibody (82880-1-RR, Clone:230065H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IHC staining of human skin cancer using 82880-1-RR

    IHC staining of human skin cancer using 82880-1-RR

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 82880-1-RR (DEK antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with DEK antibody included
  • Affinity and Kinetic Characterization of 82880-1-RR

    Affinity and Kinetic Characterization of 82880-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82880-1-RR against Human DEK were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy DEK Ready-To-Use IHC Kit. DEK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82880-1-RR targets DEK in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen DEK fusion protein Ag8225 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name DEK oncogene
Synonyms D6S231E, 230065H2
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC035259
Gene Symbol DEK
Gene ID (NCBI) 7913
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35659
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dek oncogene(DEK) is first recognized in a fusion with CAN nucleoporin protein, and it's a also autoantigen in patients with pquciarticular onset juvenile rheumatoid arthritis. As a site-specific binding protein, DEK may involve in transcriptional regulation and signal transdcution. Analysis by agarose gel celctrophoresis, DEK showed to reduce the efficiency with which chromation is replicated. It's one of the first components of the splicing complex, which remains associate with spliced exons dependent on prior splicing of pre-mRNA.

实验方案

Product Specific Protocols
WB protocol for DEK antibody 82880-1-RRDownload protocol
IHC protocol for DEK antibody 82880-1-RRDownload protocol
IF protocol for DEK antibody 82880-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols