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验证数据展示

  • WB analysis using 82886-2-RR

    WB analysis using 82886-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82886-2-RR (IFT88 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 82886-2-RR

    IF Staining of hTERT-RPE1 using 82886-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Starvation treated hTERT-RPE1 cells using IFT88 antibody (82886-2-RR, Clone: 230074E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), Acetyl-Tubulin (Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

  • FC experiment of HEK-293 using 82886-2-RR

    FC experiment of HEK-293 using 82886-2-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug IFT88 Recombinant antibody (82886-2-RR, Clone:230074E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82886-2-RR

    Affinity and Kinetic Characterization of 82886-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82886-2-RR against Human IFT88 were performed. The affinity constant is below 1 pM.

IFT88 Recombinant antibody

IFT88 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82886-2-RR

产品说明书

CloneNo. 230074E12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, A549 cells, PC-3 cells
Positive IF/ICC detected inStarvation treated hTERT-RPE1 cells
Positive FC (Intra) detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82886-2-RR targets IFT88 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 IFT88 fusion protein Ag4980 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 intraflagellar transport 88 homolog (Chlamydomonas)
别名 TTC10, TG737, Intraflagellar transport protein 88 homolog, 230074E12
计算分子量 94 kDa
观测分子量 88-95 kDa
GenBank蛋白编号BC030776
基因名称 IFT88
Gene ID (NCBI) 8100
RRIDAB_3670605
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13099
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926).

实验方案

Product Specific Protocols
WB protocol for IFT88 antibody 82886-2-RRDownload protocol
IF protocol for IFT88 antibody 82886-2-RRDownload protocol
FC protocol for IFT88 antibody 82886-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols