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MELK Recombinant antibody

MELK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230106A4

Cat no : 82893-2-RR

Print datasheet

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

验证数据展示

  • WB analysis using 82893-2-RR

    WB analysis using 82893-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 82893-2-RR

    IHC staining of mouse kidney using 82893-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 82893-2-RR

    IHC staining of mouse kidney using 82893-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82893-2-RR

    Affinity and Kinetic Characterization of 82893-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inNCCIT cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82893-2-RR targets MELK in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen MELK fusion protein Ag1897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name maternal embryonic leucine zipper kinase
Synonyms KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2
Calculated Molecular Weight 651 aa, 75 kDa
Observed Molecular Weight50-70 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14680
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

实验方案

Product Specific Protocols
WB protocol for MELK antibody 82893-2-RRDownload protocol
IHC protocol for MELK antibody 82893-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols