• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Acetyl-Histone H3 (Lys27) Recombinant antibody

Acetyl-Histone H3 (Lys27) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

1M16

Cat no : 82902-1-RR

Print datasheet

Synonyms

HIST1H3A, 1M16, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 82902-1-RR

    Dot Blot experiment of peptide using 82902-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys27) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (ug/mL) spotted is indicated next to each row. Column 1: H3K27ac. Column 2: Unmodified H3K27. Column 3: H3K9ac. Column 4: H3K14ac. Column 5: H3K18ac. Column 6: H3K23ac. Column 7: H3K36ac. Column 8: H4K5ac. Column 9: H4K8ac. Column 10: H4K12ac. Column 11: H4K16ac. Column 12: H2AK5ac. Column 13: Blank(PBS).

  • WB analysis of HeLa using 82902-1-RR

    WB analysis of HeLa using 82902-1-RR

    Various cell lysates were subjected to SDS PAGE followed by western blot with 82902-1-RR Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:9800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 82902-1-RR

    IHC staining of mouse testis using 82902-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82902-1-RR (Acetyl-Histone H3 (Lys27) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Phospho-Histone H3 (Ser10) antibody included
  • IF Staining of HeLa using 82902-1-RR

    IF Staining of HeLa using 82902-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys27) antibody (82902-1-RR, Clone: 1M16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, HSC-T6 cells, mouse kidney tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:19600
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82902-1-RR targets Acetyl-Histone H3 (Lys27) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Dot Blot, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivitymouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 1M16, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes. Acetyl-Histone H3 (Lys27) is enhancer specific mark and plays positive role in gene expression.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H3 (Lys27) antibody 82902-1-RRDownload protocol
IHC protocol for Acetyl-Histone H3 (Lys27) antibody 82902-1-RRDownload protocol
IF protocol for Acetyl-Histone H3 (Lys27) antibody 82902-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB

J Med Chem

Discovery of Novel Imidazo[1,2-a]pyridine-Based HDAC6 Inhibitors as an Anticarcinogen with a Cardioprotective Effect

Authors - Fei-Fei Yang
View Article