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KIF15 Recombinant antibody

KIF15 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230049F1

Cat no : 82903-1-RR

Print datasheet

Synonyms

KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2, hKLP2

验证数据展示

  • WB analysis using 82903-1-RR

    WB analysis using 82903-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82903-1-RR (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82903-1-RR (KIF15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82903-1-RR

    Affinity and Kinetic Characterization of 82903-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82903-1-RR against Human KIF15 were performed. The affinity constant is 75.8 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82903-1-RR targets KIF15 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen KIF15 fusion protein Ag33897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member 15
Synonyms KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2, hKLP2
Calculated Molecular Weight160 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberNM_020242
Gene Symbol KIF15
Gene ID (NCBI) 56992
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS87
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

实验方案

Product Specific Protocols
WB protocol for KIF15 antibody 82903-1-RRDownload protocol
IHC protocol for KIF15 antibody 82903-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols