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CoraLite® Plus 750-conjugated KIF15 Recombinant antibody

KIF15 Uni-rAbTM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230049F1

Cat no : CL750-82903

Print datasheet

Synonyms

KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2, hKLP2

验证数据展示

  • WB analysis using CL750-82903

    WB analysis using CL750-82903

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82903 (KIF15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells
For other applications, we recommend the unconjugated version of this antibody, 82903-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82903 targets KIF15 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen KIF15 fusion protein Ag33897 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kinesin family member 15
Synonyms KLP2, Kinesin-like protein KIF15, Kinesin-like protein 7, Kinesin-like protein 2, hKLP2
Calculated Molecular Weight160 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberNM_020242
Gene Symbol KIF15
Gene ID (NCBI) 56992
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NS87
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 KIF15 antibody CL750-82903Download protocol
Standard Protocols
Click here to view our Standard Protocols