• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82908-1-RR

    WB analysis using 82908-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82908-1-RR (SEC24A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82908-1-RR

    IF Staining of HeLa using 82908-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC24A antibody (82908-1-RR, Clone: 230105B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 82908-1-RR

    FC experiment of HepG2 using 82908-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human SEC24A (82908-1-RR, Clone:230105B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82908-1-RR

    Affinity and Kinetic Characterization of 82908-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82908-1-RR against Human SEC24A were performed. The affinity constant is below 1 pM.

SEC24A Recombinant antibody

SEC24A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82908-1-RR

产品说明书

CloneNo. 230105B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

SEC24-related protein A, Protein transport protein Sec24A, 230105B3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inU-87 MG cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82908-1-RR targets SEC24A in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SEC24A fusion protein Ag8723 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SEC24 family, member A (S. cerevisiae)
别名 SEC24-related protein A, Protein transport protein Sec24A, 230105B3
计算分子量 613 aa, 66 kDa
观测分子量120 kDa
GenBank蛋白编号BC019341
基因名称 SEC24A
Gene ID (NCBI) 10802
RRIDAB_3670637
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95486
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.

实验方案

Product Specific Protocols
WB protocol for SEC24A antibody 82908-1-RRDownload protocol
IF protocol for SEC24A antibody 82908-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols